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Darrian Bost


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Contact Information
Email: darrianbost@yahoo.com

North Carolina A&T State University, Greensboro, North Carolina 27411
Senior Undergraduate Student
Major: Chemistry
Minor: Spanish




Presentations

ABRCMS (Nov. 2010) & NC LSAMP and Rise Joint Symposium (April 2011):
  • Poster Title: Are the Neurons that Generate Breathing in Mammals Evolutionarily Conserved? Somatostatin (SST) Expression in the Caudal Medulla of Multiple Vertebrae Species.

ABRCMS (Nov. 2011), NC Opt-ED Alliance Day (October 2011) & Beta Kappa Chi (March 2011):
  • Poster Title: Evaluation of Novel Telomerase Inhibitors in Breast Cancer Cells




Research


My current research interests include:
  • Cancer Biology



Research Advisors


My advisers are Dr. Brittney-Shea Herbert (Indiana University School of Medicine) and Dr. Jessica Han (North Carolina A&T State University).

Short Bio


I am currently purisng a Bachelors degree in Chemistry at North Carolina Agricultural & Technical State University. I am also pursuing a minor study in Spanish. My career goal is to take my bench work to the bedside as a physician-scientist. I want to obtain a dual degree (M.D./Ph.D.) and I wish to work in the field of pediatrics. I think it is very valuable to have the ability to be a researcher in the laboratory and also go out in the field on the clinical side to do further research in order to find the solution to the problem. It takes a very special individual with a great personality to do this and I know I embody this quality. I want to work in the field of pediatrics because I love children and nothing would make me happier than to be able to put a smile on their faces by helping to eliminate the sickness or disease that many children endure.

My first research experience was in an anatomy and neurobiology laboratory at Washington University in St. Louis, MO School of Medicine during the summer of 2010. I spent most of my time conducting immunohistochemistry experiments, analyzing my product by fluorescent confocal imaging and cutting tissue blocks on a cryostat. I was analyzing the somatostatin expression in the caudal medulla of multiple vertebrae species to see if the neurons that generate breathing in mammals are evolutionarily conserved across this portion of the animal kingdom. Every year infants die from Sudden Infant Death Syndrome (SIDS) and this research would be helpful in identifying the neurons responsible for breathing so that further research can be done regarding those particular neurons to determine what is going wrong that kills these innocent infants. In the 10 week summer program I was not able to fully answer my question of evolution. My results showed that there may not even be a pattern of evolution across the mammal population. The next summer I worked in the lab of Dr. Brittney-Shea Herbert at Indiana University School of Medicine (IUSM)in the department of Medical and Molecular Genetics. In this lab I worked on a breast cancer project which I feel in love with. My daily work consisted of conducting telomeric repeat amplification protocols (TRAP), running PCR’s, running polyacrylamide gels and analyzing my results. My objective was to analyze the telomerase activity within various breast cancer cell lines that were untreated and treated with various concentrations of a particular drug and to determine which drug inhibited the telomerase activity the greatest. It is imperative to eliminate this enzyme from cancerous cells so they can no longer proliferate. I proved my hypothesis that as the concentration of the drug was increased the telomerase activity in the cells decreased.

I have also been privileged enough to have the opportunity to study abroad. I studied abroad during the summer of 2011 for 5 weeks after I completed conducting research at IUSM. I studied in San Jose, Costa Rica in a program entitled Medical Spanish and Healthcare in Costa Rica. I took a course where I learned medical terminology in Spanish and I took another course where I learned about the Costa Rican healthcare system and tropical diseases.


I am currently conducting academic research on campus with Dr. Jessica Han and collaborating with Dr. Marion Franks. I am about to begin a project where I will study the expression of phase 2 protiens (GST, P53, QR1) in breast cancer cells and determine the effect it may have on the cells survival. I am also looking at the iron storage in the cells and how certain drugs can upregulate the iron out of the cell.